ABSTRACT
Objective To investigate the expression of discoid domain receptor 2 (DDR2) and interferon-induced transmembrane protein 1 (IFITM1) in nasopharyngeal carcinoma (NPC) patients and their clinical significance.Methods From November 2014 to November 2015,65 patients with nasopharyngeal carcinoma were selected.All patients underwent nasopharyngeal biopsy.All specimens were embedded in paraffin after biopsy.All specimens were confirmed by pathology.Another 65 rhinitis mucosa specimens with chronic inflammation confirmed by pathology in the same period were selected as control group.The clinical and pathological data of the two groups were collected.The mRNA and protein expression levels of DDR2 and IFITM1 in nasopharyngeal carcinoma tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry.The correlation between them was analyzed by Pearson.All patients were followed up for 3 years,and the 3-year survival period of the patients was analyzed by Kaplan-Meier method.Results The results of qRT-PCR showed that the relative expression levels of DDR2 and IFITM1 in nasopharyngeal carcinoma tissues were significantly higher than those in control group (P ＜0.05).Immunohistochemical results showed that the positive rates of DDR2 and IFITM1 in nasopharyngeal carcinoma were significantly higher than those in control group (P ＜ 0.05).The results of expressions of DDR2 and IFITM1 and the clinicopathological analysis of the patients showed that the expression of DDR2 was correlated with TNM stage,differentiation degree and infiltration depth,and IFITM1 was correlated with lymph node metastasis,TNM stage,differentiation degree and infiltration depth (P ＜ 0.05).Pearson correlation analysis showed that there was correlation between DDR2 and IFITM1 (r =0.608,P ＜ 0.01).Kaplan-Meier analysis showed that the progression-free survival rate (41.17％) and total survival rate (52.94％) in DDR2 negative expression group were significantly higher than those in positive expression group (16.67％,18.75％,P ＜ 0.05).The progression-free survival rate (42.86％) and total survival rate (61.90％) in IFITM1 negative expression group were significantly higher than those in positive expression group (18.18％,20.45％,P ＜0.05).Conclusions DDR2 and IFITM1 are highly expressed in nasopharyngeal carcinoma,which may be involved in the occurrence and development of nasopharyngeal carcinoma and significantly affect the prognosis of patients.
ABSTRACT
Objective@#To investigate the expression of discoid domain receptor 2 (DDR2) and interferon-induced transmembrane protein 1 (IFITM1) in nasopharyngeal carcinoma (NPC) patients and their clinical significance.@*Methods@#From November 2014 to November 2015, 65 patients with nasopharyngeal carcinoma were selected. All patients underwent nasopharyngeal biopsy. All specimens were embedded in paraffin after biopsy. All specimens were confirmed by pathology. Another 65 rhinitis mucosa specimens with chronic inflammation confirmed by pathology in the same period were selected as control group. The clinical and pathological data of the two groups were collected. The mRNA and protein expression levels of DDR2 and IFITM1 in nasopharyngeal carcinoma tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry. The correlation between them was analyzed by Pearson. All patients were followed up for 3 years, and the 3-year survival period of the patients was analyzed by Kaplan-Meier method.@*Results@#The results of qRT-PCR showed that the relative expression levels of DDR2 and IFITM1 in nasopharyngeal carcinoma tissues were significantly higher than those in control group (P<0.05). Immunohistochemical results showed that the positive rates of DDR2 and IFITM1 in nasopharyngeal carcinoma were significantly higher than those in control group (P<0.05). The results of expressions of DDR2 and IFITM1 and the clinicopathological analysis of the patients showed that the expression of DDR2 was correlated with TNM stage, differentiation degree and infiltration depth, and IFITM1 was correlated with lymph node metastasis, TNM stage, differentiation degree and infiltration depth (P<0.05). Pearson correlation analysis showed that there was correlation between DDR2 and IFITM1 (r=0.608, P<0.01). Kaplan-Meier analysis showed that the progression-free survival rate (41.17%) and total survival rate (52.94%) in DDR2 negative expression group were significantly higher than those in positive expression group (16.67%, 18.75%, P<0.05). The progression-free survival rate (42.86%) and total survival rate (61.90%) in IFITM1 negative expression group were significantly higher than those in positive expression group (18.18%, 20.45%, P<0.05).@*Conclusions@#DDR2 and IFITM1 are highly expressed in nasopharyngeal carcinoma, which may be involved in the occurrence and development of nasopharyngeal carcinoma and significantly affect the prognosis of patients.
ABSTRACT
Objective To prospectively investigate laryngoscopy-assisted CO2 laser in the treatment of glottic laryngeal carcinoma.Methods 97 patients with early glottic laryngeal carcinoma in our hospital were selected and divided into control group (n =48) and observation group (n =49) according to random number table.The control group was treated with partial laryngectomy and the observation group was treated with laryngoscopy-assisted CO2 laser.The clinical efficacy,serum level of α1-acid glycoprotein (α1-AG) and ceruloplasmin (CER) after 10 min of the surgery and changes of the indicators of voice function [fundamental frequency jitter (jitter),the longest sound time (MPT),sound pressure level (SPL)] were observed and compared between the two groups,and the incidence of complications and quality of life (QOL) scores were statistically compared.Results The total remission rate in the observation group was 79.59％ (39/49),which was higher than that in the control group 56.25％ (27/48),with statistically significant difference (x2 =6.075,P =0.014).The levels of serum α1-AG and CER in the observation group 10 min after surgery were lower than those in the control group,with statistically significant difference (t =2.641,P =0.010,t =31.242,P =0.000).From 2 weeks after surgery to 3 months after surgery,SPL and MPT of two groups decreased first and then increased;Jitter increased first and then decreased,and the changing amplitude in the observation group was smaller than that in the control group,with statistically significant difference (P ＜ 0.05).The incidence of complications in the observation group was 2.04％ (1/49),which was lower than that in the control group 16.67％ (8/48),with statistically significant difference (P ＜ 0.05);3 months after surgery,the QOL score of the observation group was higher than that of the control group,with statistically significant difference (P ＜ 0.05).Conclusions Supporting laryngoscope CO2 laser surgery can improve stress state and quality of life of early glottic laryngeal cancer patients,and the incidence of complications is low.It has little effect on voice function.